INDIA INK PREPARATION TEST

India ink preparation test is usually employed to detect the presence of microorganisms, particularly yeast, in a body fluid. It includes negative staining by creating a black, semi opaque background that makes the capsule of the yeast visible. One drop of cerebrospinal fluid is mixed with a drop of India ink on a clean micro slide. A cover slip is placed over it and the sample is examined through the lower lens and then through the high power lens, of a microscopic. The preparation can be diluted with a small drop of water if it to dark and murky